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  • 产品名称：HIV-1Tat ELISA Kit

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  • 产品厂商：DIATHEVA
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简单介绍：
HIV-1Tat ELISA Kit
详情介绍：
Purpose Enzyme linked immunosorbent assay (ELISA) has been developed for the quantitative determination of Tat protein concentrations in biological specimens
Sample Type Serum, Plasma
Analytical Method Quantitative
Detection Method Colorimetric
Components Tat monoclonal antibody: (capture antibody) mouse anti tat antibody, 50g x 2 vials (MAINTAIN EXCLUSIVELY AT -80°C) Antigen Standard: Tat recombinant protein 0.2 ml x 2 vials (MAINTAIN AT -80°C) 96 wells plate: 12 x 8 well strips, 1 frame Plate Cover: 1 adhesive plate sealer Coating Buffer: 30ml ready-to-use Lysis Buffer: 10ml ready to use contains sodium azide Assay Diluent (BUFFER A): 50 ml ready to use Wash buffer concentrate (BUFFER B): 130ml to be diluted 10X to a final volume of 1300 with distilled water Tat Polyclonal Antibody: rabbit anti Tat antibody, 30μl Conjugated Antibody: goat anti rabbit IgG-HRP conjugated, 30μl Buffer C: 30ml ABTS tablets: 2 Buffer C1: 30μl
Material not included Microplate reader capable of measuring absorbance at 415 nm Thermomixer, shaking water bath or rocking platform at 37 °C Precision pipettes and pipette tips Glass or plastic pipettes Deionized or distilled water Multi-channel pipette, semi-automated or automated microplate washer 1000 mL graduated cylinder for preparation of 1X Wash Buffer Vortex mixer Glass tubes
Alternative Name HIV1 Tat
Application Notes Optimal working dilution should be determined by the investigator.
Comment

The detection antibody is a rabbit polyclonal anti Tat antibody. Revelation step is performed with a goat anti-rabbit HRP-conjugated antidoby, using ABTS as cromogen.The HIV-1 Tat antigenemia ELISA Kit is an aid for the quantification of HIV-1 Tat regulatory proteins.This assay is based on the sandwich ELISA technique. The detection antibody is a rabbit polyclonal anti Tat antibody. Revelation step is performed with a goat anti-rabbit HRP-conjugated antidoby, using ABTS as cromogen.
Plate Uncoated
Restrictions For Research Use only
Precaution of Use The buffer C1 provided with this kit is H2O2 solution 30% m/m (110 volumes) that causes burns
Storage 4 °C