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  • 产品名称：HIV-1Tat ELISA Kit

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  • 产品厂商：DIATHEVA
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HIV-1Tat ELISA Kit
详情介绍：
Purpose Enzyme linked immunosorbent assay (ELISA) for the quantitative determination of anti- Tat IgG concentrations in mouse serum and plasma as an aid in the diagnosis of antibody production in mouse immunized with (HIV) Tat protein
Sample Type Serum, Plasma
Analytical Method Quantitative
Detection Method Colorimetric
Components 96 wells polystyrene microplate (12 strip of 8 well ) coated with Tat protein and blocked with BSA 1% Plate Cover : 1 adhesive plate sealers Assay diluent: (Buffer A) 50 mL ready to use, with preservative Wash buffer concentrate : (Buffer B) 125 mL 10x to dilute to 1250 final volume with distilled water, with preservative. Conjugated Antibody : Goat Anti Mouse IgG-HRP conjugated 20μl Buffer C: 12.5 mL ABTS Tablets: 1 Tablet Buffer C1: 12.5 ul
Material not included Microplate reader capable of measuring absorbance at 415 nm Thermomixer, shaking water bath or rocking platform at 37 °C Precision pipettes and pipette tips Glass or plastic pipettes Deionized or distilled water Multi-channel pipette, semi-automated or automated microplate washer 1000 mL graduated cylinder for preparation of 1X Wash Buffer Vortex mixer Glass tubes
Alternative Name HIV1 Tat
Background The HIV-1 regulatory proteins Tat are considered attractive targets for the development of a multicomponent vaccine against HIV-1 infection. The protein is well conserved among different isolates and thus may be less susceptible to mutation leading to the production of escape virus variants. Tat is produced early after infection and is essential for virus replication and infectivity. Tat protein is also immunogenic and antibodies (Ab) against Tat have been found to correlate with delayed disease progression and may exert protective effects by inhibiting both HIV replication and the effects of extracellular Tat. Moreover Tat is efficiently taken up by monocite-derived dendritic cells, promotes their maturation and antigen presenting functions directing Th-1 and CTL responses against itself and other Ags since it enters the major histocompatibility complex class I pathway. Finally, vaccination of mice with a biologically active Tat protein has been shown to be safe, immunogenic and elicits anti-Tat neutralizing Ab and CTL.
Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated
Restrictions For Research Use only
Precaution of Use The buffer C1 provided with this kit is H2O2 solution 30% m/m (110 volumes) that causes burns
Storage 4 °C