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  • 产品名称：anti-MGMTantibody(O6-Methylguanine-DNA-Methyltransferase)(AA1-207)

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简单介绍：
anti-MGMTantibody(O6-Methylguanine-DNA-Methyltransferase)(AA1-207)
详情介绍：
Immunogen Purified His-tagged MGMT protein was used to produced this monoclonal antibody.
Clone 888CT22-2-4
Isotype IgG1
Purification Ascites
Alternative Name MGMT (MGMT Antibody Abstract)
Background Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Synonyms: MGMT,Methylated-DNA--protein-cysteine methyltransferase
UniProt P16455
Research Area Chromatin and Nuclear Signaling, DNA/RNA, Enzymes, Metabolism
Pathways DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
Application Notes WB: 1:2000
Restrictions For Research Use only
Format Liquid
Buffer Mouse monoclonal antibody supplied in crude ascites with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Supplier Images
Western Blotting (WB) image for anti-O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) antibody (ABIN1882265) MGMT Antibody (ABIN1882265) western blot analysis in MCF-7,Jurkat,Hela cell line lysa...
Product cited in: Edara, Goodtzova, Pegg: "The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity." in: Carcinogenesis, Vol. 16, Issue 7, pp. 1637-42, 1995 (PubMed).

Tano, Shiota, Collier, Foote, Mitra: "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 2, pp. 686-90, 1990 (PubMed).

Rydberg, Spurr, Karran: "cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells." in: The Journal of biological chemistry, Vol. 265, Issue 16, pp. 9563-9, 1990 (PubMed).

Koike, Maki, Takeya, Hayakawa, Sekiguchi: "Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase." in: The Journal of biological chemistry, Vol. 265, Issue 25, pp. 14754-62, 1990 (PubMed).

Hayakawa, Koike, Sekiguchi: "Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA." in: Journal of molecular biology, Vol. 213, Issue 4, pp. 739-47, 1990 (PubMed).