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  • 产品名称：anti-MMP2antibody(MatrixMetalloproteinase2)(N-Term)

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  • 产品厂商：半导体
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简单介绍：
anti-MMP2antibody(MatrixMetalloproteinase2)(N-Term)
详情介绍：
Immunogen Synthetic peptide derived from near N-terminus of human MMP-2 protein.
Isotype IgG
Predicted Reactivity Cow (Bovine),Chicken,Dog (Canine),Frog,Mouse (Murine),Pig (Porcine),Rabbit,Rat (Rattus)
Characteristics Cellular Localization: Cytoplasm
Purification Immunogen Affinity Purified
Background MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP-2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids. This antibody reacts with both latent and active MMP-2.
Molecular Weight 72 kDa (pro form) and approx.66 kDa (active form)
Gene ID 4313
Pathways Activation of Innate immune Response
Application Notes IHC Dilution: Ready to use, for IHC only
Comment

Positive Control:
IHC (p): Placenta, bladder, breast, ovarian carcinoma
Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 30 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Restrictions For Research Use only
Format Liquid
Buffer pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C
Supplier Images
Immunohistochemistry (IHC) image for anti-Matrix Metalloproteinase 2 (MMP2) (N-Term) antibody (ABIN1689071) anti-Matrix Metalloproteinase 2 (MMP2) (N-Term) antibody