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  • 产品名称：anti-Polymerase(DNADirected)kappa(POLK)(AA531-560),(Center)antibody

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简单介绍：
anti-Polymerase(DNADirected)kappa(POLK)(AA531-560),(Center)antibody
详情介绍：
Immunogen This POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK.
Clone RB41936
Purification This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Alternative Name POLK (POLK Antibody Abstract)
Background External and internal DNA-damaging agents continually threaten the integrity of genetic material in cells. Although a variety of repair mechanisms exist to remove the resulting lesions, some lesions escape repair and block the replication machinery. Members of the Y family of DNA polymerases, such as POLK, permit the continuity of the replication fork by allowing replication through such DNA lesions. Each Y family polymerase has a unique DNA-damage bypass and fidelity profile. POLK is specialized for the extension step of lesion bypass .

Synonyms: POLK, DINB1, DNA polymerase kappa, DINB protein
NCBI Accession NP_057302
UniProt Q9UBT6
Pathways DNA Damage Repair
Application Notes WB: 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Supplier Images
Western Blotting (WB) image for anti-Polymerase (DNA Directed) kappa (POLK) (AA 531-560), (Center) antibody (ABIN1881669) POLK Antibody (Center) (ABIN1881669) western blot analysis in 293 cell line lysates (...
Background publications Katafuchi, Sassa, Niimi, Grúz, Fujimoto, Masutani, Hanaoka, Ohta, Nohmi: "Critical amino acids in human DNA polymerases eta and kappa involved in erroneous incorporation of oxidized nucleotides." in: Nucleic acids research, Vol. 38, Issue 3, pp. 859-67, 2010 (PubMed).

Fukuda, Takamura-Enya, Masuda, Nohmi, Seki, Kamiya, Sugimura, Masutani, Hanaoka, Nakagama et al.: "Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in Vitro: REV1 inserts dC opposite the lesion, and DNA polymerase kappa potentially..." in: The Journal of biological chemistry, Vol. 284, Issue 38, pp. 25585-92, 2009 (PubMed).

Irimia, Eoff, Guengerich, Egli: "Structural and functional elucidation of the mechanism promoting error-prone synthesis by human DNA polymerase kappa opposite the 7,8-dihydro-8-oxo-2'-deoxyguanosine adduct." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22467-80, 2009 (PubMed).

Lone, Townson, Uljon, Johnson, Brahma, Nair, Prakash, Prakash, Aggarwal: "Human DNA polymerase kappa encircles DNA: implications for mismatch extension and lesion bypass." in: Molecular cell, Vol. 25, Issue 4, pp. 601-14, 2007 (PubMed).

Hanna, Kruskal, Ezekowitz, Bloom, Collier: "Role of macrophage oxidative burst in the action of anthrax lethal toxin." in: Molecular medicine (Cambridge, Mass.), Vol. 1, Issue 1, pp. 7-18, 1996 (PubMed).