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  • 产品名称：anti-GST-Tagantibody

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简单介绍：
anti-GST-Tagantibody
详情介绍：
Immunogen This GST Tag antibody is generated from rabbits immunized with a recombinant full length of GST.
Clone RB2165
Isotype Ig
Specificity This GST Tag antibody is generated from rabbits immunized with a recombinant full length of GST.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.ProtocolsProvided below are standard protocols that you may find useful for product applications.Western BlotBlocking PeptidesDot BlotImmunohistochemistryImmunofluorescence ImmunoprecipitationFlow CytometyCell Culture 
Molecular Weight 25000 DA
Research Area Tags/Labels
Application Notes WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-GST-Tag antibody (ABIN388090) The anti-GST Pab (ABIN388090) is used in Western blot to detect a GST-fusion recombin...
Product cited in: Xu, Wang, Chang, Du, Diao, Jiang, Wang, Ma, Zhang: "Mammalian sterile 20-like kinase 1/2 inhibits the Wnt/?-catenin signalling pathway by directly binding casein kinase 1?." in: The Biochemical journal, Vol. 458, Issue 1, pp. 159-69, 2014 (PubMed).

Rajyaguru, She, Parker: "Scd6 targets eIF4G to repress translation: RGG motif proteins as a class of eIF4G-binding proteins." in: Molecular cell, Vol. 45, Issue 2, pp. 244-54, 2012 (PubMed).

Nissan, Rajyaguru, She, Song, Parker: "Decapping activators in Saccharomyces cerevisiae act by multiple mechanisms." in: Molecular cell, Vol. 39, Issue 5, pp. 773-83, 2010 (PubMed).