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  • 产品名称：anti-MBD1antibody(Methyl-CpGBindingDomainProtein1)(AA376-405)

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简单介绍：
anti-MBD1antibody(Methyl-CpGBindingDomainProtein1)(AA376-405)
详情介绍：
Immunogen This MBD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-405 amino acids from the C-terminal region of human MBD1.
Clone RB2371
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Purified Rabbit Polyclonal Antibody (Pab)
Alternative Name MBD1 (MBD1 Antibody Abstract)
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).

Synonyms: MBD1, CXXC3, PCM1, Methyl-CpG-binding domain protein 1, CXXC-type zinc finger protein 3, Methyl-CpG-binding protein MBD1, Protein containing methyl-CpG-binding domain 1
NCBI Accession NP_001191071, NP_001191065, NP_001191070, NP_001191066, NP_001191072, NP_056669, NP_001191080, NP_001191069, NP_001191067, NP_001191068, NP_002375, NP_056670, NP_056671, NP_056723
UniProt Q9UIS9
Research Area Chromatin and Nuclear Signaling
Application Notes WB: 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Supplier Images
Western Blotting (WB) image for anti-Methyl-CpG Binding Domain Protein 1 (MBD1) (AA 376-405), (C-Term) antibody (ABIN1882100) Western blot analysis of anti-MBD1 Pab (ABIN1882100) in HeLa cell lysate. MBD1 (Arrow...
Product cited in: Auriol, Billard, Magdinier, Dante: "Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus." in: Nucleic acids research, Vol. 33, Issue 13, pp. 4243-54, 2005 (PubMed).

Background publications Bader, Walker, Harrison: "Most microsatellite unstable sporadic colorectal carcinomas carry MBD4 mutations." in: British journal of cancer, Vol. 83, Issue 12, pp. 1646-9, 2001 (PubMed).

Petronzelli, Riccio, Markham, Seeholzer, Stoerker, Genuardi, Yeung, Matsumoto, Bellacosa: "Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32422-9, 2000 (PubMed).

Bhattacharya, Ramchandani, Cervoni, Szyf: "A mammalian protein with specific demethylase activity for mCpG DNA." in: Nature, Vol. 397, Issue 6720, pp. 579-83, 1999 (PubMed).

Hendrich, Bird: "Identification and characterization of a family of mammalian methyl-CpG binding proteins." in: Molecular and cellular biology, Vol. 18, Issue 11, pp. 6538-47, 1998 (PubMed).