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产品名称:Mouseanti-Cow(Bovine)IgG(LightChain)Antibody
产品型号:
产品厂商:半导体
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简单介绍:
Mouseanti-Cow(Bovine)IgG(LightChain)Antibody
详情介绍:
Product details
Product details
Immunogen
Fraction of Bovine immunoglobulin.
Clone
IVA285-1
Isotype
IgG1
Specificity
The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Purification
Purified from ascites by protein-A affinity chromatography.
Purity
> 95 % (by SDS-PAGE)
Target details
Target details
Research Area
Immunology, Secondary Antibodies
Application Details
Application Details
Application Notes
Western Blotting: Recommended dilution: 0,5-1 µg/mL, overnight in 4 °C
Positive control and sample preparation: BS (bovine serum), dilution 1: 50 in Laemmli reducing buffer, boiled in water bath for 3 min
Application notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM.Reducing conditions. SDS-PAGE (12 % separating gel).
Flow Cytometry:
Working concentrations should be determined by the investigator.
Restrictions
For Research Use only
Handling
Handling
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Do not freeze.
Storage
4 °C
Storage Comment
Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Images
Images
Supplier Images
Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and B...
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Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).
Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and Bovine Serum (right lane) by antibody IVA285-1. This antibody is a suitable tool for in-house quality controls of Fetal Calf Serum (Hybridoma Cell Culture grade).
References
References
Product cited in:
Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin."
in:
Hybridoma (2005)
, Vol. 25, Issue 5, pp. 309-12, 2006 (PubMed).