Western Blot of Peroxidase conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. Lane 2: Human IgG. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:5,000 for 60 min at RT. Block: 5% BSA-TBS overnight at 4°C. Predicted/Observed size: 55 kDa, 28 kDa for Goat IgG. Other band(s): none.