Goatanti-RabbitIgG(Heavy&LightChain)Antibody(HRP) 抗体网

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产品名称：Goatanti-RabbitIgG(Heavy&LightChain)Antibody(HRP)
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简单介绍：

Goatanti-RabbitIgG(Heavy&LightChain)Antibody(HRP)

详情介绍：

Product details
Product details
Immunogen	Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in goat.
Isotype	IgG
Specificity	IgG (H&L)
Characteristics	Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm

Target details
Target details
Background	Synonyms: Rabbit Antibody peroxidase conjugated, Gt-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in Goat, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Restore with deionized water (or equivalent)
Concentration	2.0 mg/mL
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	Gentamicin sulfate
Handling Advice	Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Images
Images
Supplier Images	Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody. Lane 1: HeLa Who... Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (Image 2) Images for product: Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH 3T3 Whole Cell Lysate. Load: 10 µg per lane. Primary antibody: Beta Actin antibody at 1:2,000 for overnight at 4°C. Secondary antibody (2 Blots on Left): Atto 647N goat secondary antibody at 1:10,000 for 60 min at RT. Secondary Antibody (2 Blots on Right): Peroxidase goat secondary antibody at 1:10,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 50 kDa, 50 kDa for Beta Actin. Other band(s): none. Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (Image 2)

References
References
Background publications	Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).