Western Blot of Chicken anti-Human IgG (H&L) Antibody Peroxidase Conjugated. Lane 1: Human IgG. Load: 100 ng per lane. Secondary antibody: Human IgG (H&L) Antibody Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.