Goatanti-MonkeyIgG(Chaingamma)Antibody(Rhodamine) 抗体网

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产品名称：Goatanti-MonkeyIgG(Chaingamma)Antibody(Rhodamine)
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产品厂商：半导体
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简单介绍：

Goatanti-MonkeyIgG(Chaingamma)Antibody(Rhodamine)

详情介绍：

Product details
Product details
Immunogen	Monkey IgG gamma heavy chain
Isotype	IgG
Specificity	IgG (gamma chain)
Characteristics	Concentration Definition: by UV absorbance at 280 nm

Target details
Target details
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Restore with deionized water (or equivalent)
Concentration	1.0 mg/mL
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	Sodium azide
Precaution of Use	WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice	Product is photosensitive and should be protected from light.

Images
Images
Supplier Images	Western Blot showing detection of Monkey IgG Gamma Chain. 100ng of Monkey IgG (Lane 2... Western Blot showing detection of Monkey IgG Gamma Chain. 100ng of Monkey IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 5% Blotto (p/n B501-0500) 30 min at 20°C, Anti-Monkey IgG (gamma chain) (GOAT) Antibody Rhodamine Conjugated (p/n 617-100-012) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP. Molecular weight markers are in lane 1. Western Blot showing detection of Monkey IgG Gamma Chain. 100ng of Monkey IgG (Lane 2) was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 5% Blotto (p/n B501-0500) 30 min at 20°C, Anti-Monkey IgG (gamma chain) (GOAT) Antibody Rhodamine Conjugated (p/n 617-100-012) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP. Molecular weight markers are in lane 1.

References
References
Background publications	Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).