Rabbitanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(HRP) 抗体网

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产品名称：Rabbitanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(HRP)
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简单介绍：

Rabbitanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(HRP)

详情介绍：

Product details
Product details
Immunogen	Mouse IgG whole molecule
Isotype	IgG
Specificity	IgG (H&L)
Characteristics	Concentration Definition: by UV absorbance at 280 nm

Target details
Target details
Background	Synonyms: mouse antibody, anti mouse igg conjugated secondary antibody
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	Optimal working dilutions should be determined experimentally by the investigator.
Comment	When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Restore with deionized water (or equivalent)
Concentration	2.0 mg/mL
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	Gentamicin sulfate
Handling Advice	Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Images
Images
Supplier Images	Western Blot of Anti-Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mo... Western Blot of Anti-Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 µm pore size)., and blocked with 5% BLOTTO (p/n B501-0500) o/n at 4° C. The membrane was then probed with a 1:2,500 dilution of HRP Rb-a-Mouse IgG [H&L] (p/n 610-4302) for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX-110). Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www.uvp.com, Upland, CA). Binning: 5X5. Exposure time: 2 min. Aperture open. No emission or excitation filters used. Western Blot of Anti-Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 µm pore size)., and blocked with 5% BLOTTO (p/n B501-0500) o/n at 4° C. The membrane was then probed with a 1:2,500 dilution of HRP Rb-a-Mouse IgG [H&L] (p/n 610-4302) for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate (p/n FEMTOMAX-110). Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www.uvp.com, Upland, CA). Binning: 5X5. Exposure time: 2 min. Aperture open. No emission or excitation filters used.

References
References
Background publications	Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).