Goatanti-RabbitIgG(Heavy&LightChain)Antibody 抗体网

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产品名称：Goatanti-RabbitIgG(Heavy&LightChain)Antibody
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产品厂商：半导体
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简单介绍：

Goatanti-RabbitIgG(Heavy&LightChain)Antibody

详情介绍：

Product details
Product details
Immunogen	Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in goat.
Isotype	IgG
Specificity	IgG (H&L)
Characteristics	Anti-Rabbit IgG antibody generated in goat detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
Sterility	Sterile filtered

Target details
Target details
Background	Synonyms: Rabbit Antibody, Gt-a-Rabbit IgG, Rabbit Antibody in Goat, Rabbit Secondary Antibody
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	Anti-Rabbit IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Restore with deionized water (or equivalent)
Concentration	1.0 mg/mL
Buffer	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative	Sodium azide
Precaution of Use	WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Images
Images
Supplier Images	Western Blot of Goat anti-RABBIT IgG (H&L) Antibody Pre-adsorbed. Lane 1: Rabbit IgG.... Western Blot of Goat anti-RABBIT IgG (H&L) Antibody Pre-adsorbed. Lane 1: Rabbit IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Rabbit IgG. Other band(s): none. Western Blot of Goat anti-RABBIT IgG (H&L) Antibody Pre-adsorbed. Lane 1: Rabbit IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Rabbit IgG. Other band(s): none.

References
References
Background publications	Titus, Haugland, Sharrow, Segal: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: Journal of immunological methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).