Application Notes
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The stated application concentrations are suggested starting points. Titration of the recombinant Mouse IgG2a-Kappa antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 0.005 μg/mL - 0.2 μg/mL,Immunocytochemistry: 0.5-2 μg/mL,Immunohistochemistry (FFPE): 0.5-2 μg/mL (1),Western Blot (non-reduced only): 0.1-0.5 μg/mL
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