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  • 产品名称：Mouseanti-HumanIgMHeavyChainAntibody

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  • 产品厂商：NSJBio
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简单介绍：
Mouseanti-HumanIgMHeavyChainAntibody
详情介绍：
Immunogen Recombinant heavy chain of human IgM was used as the immunogen for this antibody.
Clone IM373
Isotype IgG1, kappa
Characteristics This antibody recognizes a protein of 75 kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Purification Protein G affinity chromatography
Background This antibody recognizes a protein of 75?kDa, identified as the mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Gene ID 3507
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10?mM citrate buffer,?pH 6.0, for 10-20?min followed by cooling at RT for 20?minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30?min.\. FACS: 0.5-1?μg/million cells,IF: 0.5-1?μg/mL,WB: 0.5-1?μg/mL,IHC (Frozen): 0.5-1?μg/mL for 30?minutes at RT (1),Prediluted format : incubate for 30?min at RT (2)
Restrictions For Research Use only
Buffer 1?mg/mL in 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store the IgM heavy chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).