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  • 产品名称：Goatanti-Mouse(Murine)IgG2b(HeavyChain)Antibody(IRDye680LT)

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  • 产品厂商：LI-COR
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简单介绍：
Goatanti-Mouse(Murine)IgG2b(HeavyChain)Antibody(IRDye680LT)
详情介绍：
Immunogen Mouse IgG2b paraproteins
Isotype IgG
Fragment Whole molecule
Specificity Mouse IgG2b
Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2b.
Cross-Reactivity (Details) This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgM, IgG1, IgG2a, IgG3, IgA, pooled human sera, and purified human paraproteins.
Purification Immunoaffinity chromatography
Research Area Secondary Antibodies
Application Notes Western blot: 1:20,000 Recommended
Optimum dilutions will vary and should be determined empirically.

NOTE: When using PVDF membranes for Western blotting, SDS (final concentration of 0.1 – 0.2%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.

  • IRDye 680LT secondary antibodies are suitable for immunofluorescent microscopy and other fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
  • IRDye 680LT is not intended for In-Cell WesternTM assays or in vivo applications.
  • These antibodies are not recommended when getting up and running on system. Once established near-infrared protocols are optimized with IRDye 680RD, IRDye 680LT can be used to optimize signals in the 700 channel. Dilution range 1:20,000 – 1:40,000.
    Note: optimization may be required with IRDye 680LT. Please see pack insert for specific detergents/protocol modifications needed.
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    Comment

    • IRDye 680LT secondary antibodies have been proven to provide the brightest signal for Western blot detection in the 700 nm channel and are comparable to Alexa Fluor® 680 secondary antibodies.
    • Choose IRDye 680LT secondary antibodies to get high signal and for specific uses of detection in the 700nm channel.
    • We do not recommend to start with IRDye 680LT secondaries but rather to start with one of the IRDye 680RD secondaries.

    Restrictions For Research Use only
    Format Lyophilized
    Reconstitution Reconstitute with sterile, distilled water
    Concentration 1.0 mg/mL
    Buffer PBS, pH 7.4
    Preservative Sodium azide
    Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice Protect from light.
    Storage 4 °C
    Product cited in: Mehuron, Kumar, Duarte, Yamauchi, Accorsi, Girgenrath: "Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy." in: Skeletal muscle, Vol. 4, pp. 14, 2014 (PubMed).