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产品名称:Goatanti-Mouse(Murine)IgG2b(HeavyChain)Antibody(IRDye680LT)
产品型号:
产品厂商:LI-COR
产品文档:
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简单介绍:
Goatanti-Mouse(Murine)IgG2b(HeavyChain)Antibody(IRDye680LT)
详情介绍:
Product details
Product details
Immunogen
Mouse IgG2b paraproteins
Isotype
IgG
Fragment
Whole molecule
Specificity
Mouse IgG2b
Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2b.
Cross-Reactivity (Details)
This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with mouse IgM, IgG1, IgG2a, IgG3, IgA, pooled human sera, and purified human paraproteins.
Purification
Immunoaffinity chromatography
Target details
Target details
Research Area
Secondary Antibodies
Application Details
Application Details
Application Notes
Western blot: 1:20,000 Recommended
Optimum dilutions will vary and should be determined empirically.
NOTE: When using PVDF membranes for Western blotting, SDS (final concentration of 0.1 – 0.2%) and Tween® 20 (final concentration of 0.1 – 0.2%) must be added during the detection incubation step to avoid non-specific background staining.
IRDye 680LT secondary antibodies are suitable for immunofluorescent microscopy and other fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities.
IRDye 680LT is not intended for In-Cell Western
TM
assays or in vivo applications.
These antibodies are not recommended when getting up and running on system. Once established near-infrared protocols are optimized with IRDye 680RD, IRDye 680LT can be used to optimize signals in the 700 channel. Dilution range 1:20,000 – 1:40,000.
Note: optimization may be required with IRDye 680LT. Please see pack insert for specific detergents/protocol modifications needed.
Comment
IRDye 680LT secondary antibodies have been proven to provide the brightest signal for Western blot detection in the 700 nm channel and are comparable to Alexa Fluor® 680 secondary antibodies.
Choose IRDye 680LT secondary antibodies to get high signal and for specific uses of detection in the 700nm channel.
We do not recommend to start with IRDye 680LT secondaries but rather to start with one of the IRDye 680RD secondaries.
Restrictions
For Research Use only
Handling
Handling
Format
Lyophilized
Reconstitution
Reconstitute with sterile, distilled water
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Protect from light.
Storage
4 °C
References
References
Product cited in:
Mehuron, Kumar, Duarte, Yamauchi, Accorsi, Girgenrath: "Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy."
in:
Skeletal muscle
, Vol. 4, pp. 14, 2014 (PubMed).