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产品名称:Goatanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(IRDye680RD)
产品型号:
产品厂商:LI-COR
产品文档:
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简单介绍:
Goatanti-Mouse(Murine)IgG(Heavy&LightChain)Antibody(IRDye680RD)
详情介绍:
Product details
Product details
Brand
In-Cell Western™,CellTag™
Immunogen
Mouse IgG paraproteins
Isotype
IgG
Fragment
Whole molecule
Specificity
Mouse IgG
Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA.
Cross-Reactivity (Details)
This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species.
Purification
immunoaffinity chromatography
Target details
Target details
Research Area
Immunology, Secondary Antibodies
Application Details
Application Details
Application Notes
Western blot: 1:5,000 - 1:25,000
Restrictions
For Research Use only
Handling
Handling
Format
Lyophilized
Reconstitution
Reconstitute with sterile, distilled water
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Protect from light.
Storage
4 °C
Images
Images
Supplier Images
Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with m...
Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with se...
The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-M...
Levels of GAD67, beta-III tubulin, and GAPDH in primary neurons. Primary neurons were...
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Images for product: Goat anti-Mouse (Murine) IgG (Heavy & Light Chain) antibody (IRDye680RD)
Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with mouse anti-alpha-tubulin and rabbit anti-ELF5 followed by detection with IRDye® 680RD Goat anti-Mouse IgG and IRDye 800CW Goat anti-Rabbit IgG.
Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with serial dilutions of EGF (6.3-200 ng/mL). Lysates were resolved by SDS-PAGE and transferred to Odyssey® Nitrocellulose Membrane. The blot was probed with rabbit anti-phosphoEGFR and mouse anti-ERK2 followed by detection with IRDye® 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-Mouse IgG.
The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-Mouse IgG
Levels of GAD67, beta-III tubulin, and GAPDH in primary neurons. Primary neurons were genetically manipulated with a Cre-lox system to delete apoE4, and lysates were prepared. GAD67 and GAPDH were detected with mouse primary antibodies and IRDye® 800CW Goat anti-Mouse IgG (green bands. Beta-III tubulin was detected with rabbit primary and IRDye 680RD Goat anti-Rabbit IgG (red bands).
References
References
Product cited in:
Mitra, Goodman, Le: "Enhanced detection of metastatic prostate cancer cells in human plasma with lipid bodies staining."
in:
BMC cancer
, Vol. 14, pp. 91, 2014 (PubMed).