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  • 产品名称:Z-AEVD-FMK(Caspase-10Inhibitor)

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  • 产品厂商:KamiyaBiomedical
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简单介绍:
Z-AEVD-FMK(Caspase-10Inhibitor)
详情介绍:
Purpose Caspase-6, 8, 9 Inhibitor 
Sequence Z-Ala-Glu(Ome)-Val-Asp(Ome)-FMK
Specificity FMK inhibitors based on the sequence AEVD will inactivate caspases-6, 8, 9, and to a lesser extent caspases-1 and 3. Caspases-2, 4, 5, 7, and 10 will be little affected.
Formula C₂₈H₃₉N₄O₁₀F
Solubility Soluble in both DMSO and water.
Molecular Weight 610 Da
Comment

Lyophilized solid. Peptide-fluoromethyl ketone inhibitor of caspases.
The CH2F (fluoromethyl ketone) inhibitor has several advantages over other types of derivatives: Penetrates cell membranes, Not toxic to cells, Irreversible inhibition
Available data indicates that FMK inhibitors based on the sequence AEVD will inactivate caspases-6, 8, 9, and to a lesser extent caspases-1 and 3. Caspases-2, 4, 5, 7, and 10 will be little affected.
Dissolve the Caspase-13 Inhibitor in DMSO before use. It is important that dry, good quality DMSO be used.

For use on intact cells:
1. Prepare desired concentrated stock solutions as follows:
3 mg Z-AEVD-FMK
in 24µl DMSO = 20 mM
in 49µl DMSO = 10 mM
in 98µl DMSO = 5 mM, etc.

2. Add 2 µl of above stock solution to 1 ml culture medium containing cells such that the fµl DMSO concentration is 0.2%. Levels of DMSO above this may be toxic, thus masking the effect of the Caspase-13 protease inhibitor. Adding 2 µl of a 10 mM stock solution to 1 ml of culture medium gives a final Z-AEVD-FMK concentration of 20 µM. Typical final concentrations are 5 - 20 µM.

For extended use in vivo and in vitro:
For experiments extending 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation of the inhibitor by endogenous cysteine proteases.

Restrictions For Research Use only
Format Lyophilized
Storage RT