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  • 产品名称：Z-DEVD-FMK(Caspase-3Inhibitor)

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  • 产品厂商：KamiyaBiomedical
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简单介绍：
Z-DEVD-FMK(Caspase-3Inhibitor)
详情介绍：
Purpose Irreversible caspase-1, 3, 4, 7, 8, 10 inhibitor 
Sequence Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-CH2F, Z-DEVD-FMK
Characteristics Levels of DMSO above 0.2% may cause some cellular toxicity, thus masking the effect of the protease inhibitor. For extended in vitro and in vivo use: For experiments extending 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation of the inhibitor by endogenous cysteine proteases. IMPORTANT NOTE for in vitro use: Our peptide inhibitors are synthesized as methyl esters to enhance cell permeability. In intact cells, the methyl groups are removed by endogenous enzymes. For in vitro experiments with purified enzymes, however, the methyl groups must first be removed by treating the inhibitor with esterase. A procedure is available upon request.
Chemical Name Z-DEVD-fluoromethylketone
Formula C₃₀H₄₁FN₄O₁₂
Solubility DMSO
Molecular Weight 668 Da
Application Notes Inhibition of caspase-3, -7, -1, -4, -8, and -10 activities. For caspase-3 fluorometric assays using the Caspase-3 Fluorogenic Substrate (Cat. No. AC-003), Caspase-3(CPP32) Inhibitor can be used to assess the contribution of contaminating proteases to the overall rate of proteolysis.
Comment

Irreversible and cell permeable inhibitor of caspase-3 (CPP32, Apopain), a member of the CIE/CED-3 family of cysteine proteases. The CH2F (fluoromethyl ketone) inhibitor has several advantages over other types of derivatives: Penetrates cell membranes, is nontoxic to cells, irreversible inhibition.
Caspase-3 is a member of the cysteine proteases family involved in apoptosis induction. All apoptotic pathways studied to date involve proteolytic activation of caspase-3 as a central event in the progression of cell death. Although the death-inducing consequences of caspase-3 activation have not been conclusively established, several crucial substrates for the protease have been identified in vitro, including DNA-dependent protein kinase, Poly(ADPribose) Polymerase (PARP), Replication factor C, and Gelsolin. These substrates are involved in the later stages of apoptosis, strongly suggesting that caspase-3 has a key role in promoting the final processes leading to cell death.
Inhibition of caspase-3, -7, -1, -4, -8, and -10 activities. For caspase-3 fluorometric assays using the Caspase-3 Fluorogenic Substrate (ABIN924940), Caspase-3(CPP32) Inhibitor can be used to assess the contribution of contaminating proteases to the overall rate of proteolysis.
Dissolve the Caspase-3(CPP32) Inhibitor in high purity DMSO (>99.9%) before use to make a stock solution of 20 mM.

For use on intact cells:
1. Prepare desired concentrated stock solutions as follows:
5 mg Z-DEVD-FMK
in 37µl DMSO = 20 mM
in 75µl DMSO = 10 mM
in 1,50µl DMSO = 5 mM, etc.

2. Adding 2 µL of the above stock solutions to 1 mL of culture medium containing cells gives a fµl DMSO concentration of 0.2%. Adding 2 µL of a 10 mM stock solution to 1 mL of culture medium gives a final Z-DEVD-FMK concentration of 20 µM.
Restrictions For Research Use only
Format Solid
Storage RT