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  • 产品名称：Biotin-FA-FMK(CathepsinBInhibitor)

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  • 产品厂商：KamiyaBiomedical
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简单介绍：
Biotin-FA-FMK(CathepsinBInhibitor)
详情介绍：
Purpose Irreversible cathepsin B inhibitor. Does not bind to or inhibit any of the caspases
Sequence Biotin-Phe-Ala-CH2F
Specificity Specifically binds to and inhibits Cathepsin B. Does not bind to or inhibit any of the caspases.
Characteristics Trifluoroacetic acid salt of the fluoromethyl ketone biotinylated peptide inhibitor of Cathepsin B (Caspase inhibitor negative control). The CH2F (fluoromethyl ketone) inhibitor has several advantages over other types of derivatives:
- Penetrates cell membranes
- Not toxic to cells
- Irreversible inhibition
The ten known caspases are involved in theproteolytic cascade that results in apoptosis andin the maturation process of IL-1ß necessary forinflammation. The use of biotinylated FMK-typeinhibitors for various caspases requires abiotinylated FMK-type inhibitor that does notaffect caspases as a negative control. TheBiotin-FA-FMK does not inhibit the activity ofcaspases in intact cells and does not block theinduction of apoptosis as caspase inhibitors do.It can, therefore, be used as a negative controlfor the FMK moiety of the biotinylated caspaseinhibitor, both in vivo and in vitro.
Solubility DMSO
Molecular Weight 478 Da
Application Notes Irreversible binding to and inhibition of Cathepsin B. The second order rate constant (k2) of inactivation for human liver Cathepsin B is 1,220 M -1 s -1 , determined from the observed rate of inactivation (k) divided by the inhibitor concentration (I): k2 = k/I. Serves as a negative control in studies using the biotinylated FMK-containing caspase inhibitors.
Comment

Dissolve Biotin-FA-FMK in high purity (>99.9%) DMSO before use.

For use on intact cells:
1. Prepare desired concentrated stock solution in DMSO as follows:
Dissolve 1 mg Biotin-FA-FMK
in 68 µl DMSO = 25 mM
in 85 µl DMSO = 20 mM
in 169 µl DMSO = 10 mM, etc.

2. Add 2 µL of the stock solution to 1 mL culture medium containing cells such that the final DMSO concentration is 0.2%. Levels of DMSO above this may cause some cellular toxicity, thus masking the true effects of the inhibitor. The recommended final concentration of Biotin-FA-FMK for use as a caspase inhibitor negative control is 50 µM (2 µL of 25 mM stock solution in 1 mL cell culture).

3. Detect bound Biotin-FA-FMK by any of several avidin/streptavidin methods.

For extended use in vivo or in vitro:
For experiments extending 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation of the inhibitor by endogenous cysteine proteases.
Restrictions For Research Use only
Format Solid
Storage RT