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  • 产品名称：Caspase-8Substrate-pNA

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  • 产品厂商：KamiyaBiomedical
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简单介绍：
Caspase-8Substrate-pNA
详情介绍：
Purpose Soluble in DMSO and aqueous buffers. We recommend preparing a stock solution in high purity (>99.9%) DMSO, and diluting into aqueous buffer shortly prior to use.
1. Lyse cells in 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT, at a density of 2 x 10^6 /mL.
2. Assay 0.01 mL cell lysate in a final volume of 0.1 mL. Assay buffer is cell lysis buffer containing 0.2 mM substrate.
3. Incubate at 37°C for 0-3 hr. Take periodic readings of absorbance at 405 nm.
Sequence Acetyl-Ile-Glu-Thr-Asp-pNA, Ac-IETD-pNA
Specificity Substrate for caspase-8. Also cleaved by granzyme B.
Purity 98 +/- 1 % by HPLC.
Background Chromogenic paranitroanilide- peptide substrate for caspase-8. Also cleaved by granzyme B. Release of free pNA is monitored by absorbance at 405 nm (epsilon=9,160 M^-1 cm^-1 ). Caspase-8/FLICE (also known as MACH or Mch5) is a member of the caspase family of cysteine proteases. Caspases play an important role in apoptosis signaling and effector mechanisms. Caspase-8 is most similar to caspase-10, both of which have death domain motifs. Caspase-8 appears to be physically associated with the signaling mechanism during Fas-mediated cell death and its association with Fas or tumor necrosis factor receptors via an interaction with FADD suggests it functions as an initiator rather than an effector of the cell death pathway. Thus, Caspase-8 is an upstream activator in the protease cascade that proteolytically matures other caspases.
Molecular Weight 638 Da
Application Notes Assay of caspase activity in cell extracts.
Comment

Formula: C27H38N6O12
Restrictions For Research Use only
Format Lyophilized
Storage RT