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Comment
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This buffer is applicable to most standard immunoprecipitation procedures. It is first used to lyse cells, followed by the immunoprecipitation of assembled biomolecules. The addition of antibody for immunoprecipitation, subsequent addition of immunoprecipitation beads, such as G-Sepharose, pull down and elution is the general strategy used to reveal direct or in-direct interactions between proteins using this reagent. Triton abrogates hydrophobic interactions, but preserves charge-charge and hydrogen bonding interactions, which are captured and preserved in the immunoprecipitation proceudre. Activity assays or Western blotting analysis are subsequently employed to detect the assembled biomolecular complex.
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