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Specificity
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6-FAM Phosphoramidite (FAM-amidite, FAM Phosphoramidite) is employed to label oligonucleotides with fluorescein. It has applicability in detection of nucleotide hybridization, changes in nucleotide conformation, transcription factor binding to DNA, tracing of siRNA introduction into cells, quenching interactions between labeled oligonucleotides with interacting binding molecules, and quantitation of PCR amplification. FIVEphoton Biochemicals offers 6-FAM Phosphoramidite as a core product in its oligonucleotide synthesis and modification reagent portfolio.
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