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  • 产品名称:CHAPSBuffer

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  • 产品厂商:FIVEphoton
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简单介绍:
CHAPSBuffer
详情介绍:
Characteristics Strong cell lysis and protein extraction capacity while preserving protein conformation and protein-protein interaction for co-immunoprecipitation (Co-IP) analysis.
Effective as a stand alone reagent for cell lysis and protein extraction.
Contains a non-amine buffer enabling cross-linking with NHS-ester derivatives.
Minimal interference with Bradford and Lowry protein assays.
60 mL volume: Suitable for two hundred 10-cm culture plates, or 600 wells in 6-well dishes.
Comment

Properties of CHAPS Lysis and Immunoprecipitation BufferCHAPS is a zwitterionic detergent with strong membrane dissolution capacity while maintaining protein conformation and protein-protein interaction. CHAPS reagent provides an effective stand alone buffer for cell lysis and protein extraction as well as a reagent suitable for downstream immunoprecipitation-pull down approaches. The standard FIVEphoton Biochemicals CHAPS reagent is provided without calcium chloride or EDTA.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 %
Buffer CHAPS based cell lysis and immunoprecipitation buffer containing 0.5% CHAPS, pH 8.0.
Storage 4 °C