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  • 产品名称：EstroneSulfate ELISA Kit

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  • 产品厂商：国内供应2
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EstroneSulfate ELISA Kit
详情介绍：
Purpose This ELISA Kit applies to the in vitro quantitative determination of E1S concentrations in serum, plasma and other biological fluids.
Sample Type Plasma, Serum, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This kit recognizes natural and recombinant E1S. No signif icant cross-reactivity or interference between E1S and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between E1S and all the analogues.
Sensitivity 1.875 ng/mL
Alternative Name Estrone Sulfate (E1S)
Sample Volume 100 μL
Plate Pre-coated
Protocol This ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with E1S. During the reaction, E1S in the sample or standard competes with a fixed amount of E1S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E1S. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E1S in the samples is then determined by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
Restrictions For Research Use only
Storage Comment All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20°C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur. 6th Edition, revised in June, 2015 www.elabscience.com 3