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产品名称:Rabbitanti-Pig(Porcine)IgGAntibody(Biotin)
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产品厂商:Cloud-Clone
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简单介绍:
Rabbitanti-Pig(Porcine)IgGAntibody(Biotin)
详情介绍:
Product details
Product details
Immunogen
Native Protein IgG Immunoglobulin G (IgG) is one of the most abundant proteins in serum and the major components of the immune system. IgG is important for our defence against microorganisms and the molecules, which are produced by B lymphocytes as a part of our adaptive immune response. By binding many kinds of pathogen—representing viruses, bacteria, and fungi—IgG protects the body from infection. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
The antibody is a rabbit polyclonal antibody raised against IgG conjugated to biotin.
Isotype
IgG
Specificity
It has been selected for its ability to recognize IgG in immunohistochemical staining and Western blotting.
Purification
Affinity Chromatography
Target details
Target details
Research Area
Immunology, Secondary Antibodies
Application Details
Application Details
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Restrictions
For Research Use only
Handling
Handling
Format
Liquid
Concentration
200 µg/mL
Buffer
Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid repeated freeze/thaw cycles
Storage
4 °C
Storage Comment
Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date
12 months
References
References
Product cited in:
Benkahla, Sane, Desailloud, Hober: "Exposure of Piglets to Enteroviruses Investigated by an Immunoassay Based on the EV-G1 VP4 Peptide."
in:
Intervirology
, Vol. 59, Issue 2, pp. 69-73, 2016 (PubMed).