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  • 产品名称：Mouseanti-RabbitIgGAntibody(Beads)

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  • 产品厂商：BosterBio
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简单介绍：
Mouseanti-RabbitIgGAntibody(Beads)
详情介绍：
Isotype IgG
Characteristics Mouse Anti-Rabbit Beads contains sepharose beads coated with mouse anti-rabbit IgG, for purification of rabbit IgG class of antibodies or proteins with affinity to mouse anti-rabbit IgG. The mouse anti-rabbit IgG is affinity purified and conjugated to the beads at 1-1. 5 mg/mL ratio. This product can be used for 100-200 times. It may also be used for immunoprecipitation (IP).

Mouse Anti-Rabbit Beads Specifications
Matrix: CNBr-activated SepharoseTM 4FF.
Beads concentration: 1-1. 5 mg/mL.
Coupling conditions of matrix: pH 7-9, 4 °C to 25 °C, 2-16 h.
Binding capacity: 4-7 mg IgG per mL.
Bead size range: 45-165 µm.
Mean bead size: 90 µm.
Bead structure: Highly cross-linked agarose, 4 %.
Max. flow rate: 4 mL/min/cm^2.
Recommended flow rate: 1-3 mL/min/cm^2.
Stability of the matrix: pH 3-11 (ligand dependent).
Purification Affinity purification
Research Area Immunology, Secondary Antibodies
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Protein Purification Beads
Reagent Preparation

Buffers preparation:
Equilibration buffer A: 1 % NaCl+0.1 % Na2HPO4, pH 7.5.
Equilibration buffer B: 1 % CH3COONa adjusted pH to 5 by CH3COOH.
Elution buffer: 2 % table sugar adjusted pH to 2-3 by CH3COOH.
Wash buffer: purified water.
Sample Preparation

Sample preparation:
1. Dilute the serum with equilibration buffer A to ensure its content and pH closed to equilibration buffer A.
2. Centrifuge diluted serum supernatants to sediment debris.
3. Filter supernatants through 0.45 nm filter.
Assay Procedure

Affinity-purification:
1. Load the Mouse Anti-Rabbit beads into the empty column.
2. Wash column with Wash buffer in 3-5 column volumes to remove the glycerol, and then, equilibrate column by washing with Equilibration buffer A in 5-10 column volumes.
3. Bring the sample to room temperature, and load it into the column by a syringe or a pump. The total volume of the sample applied is not critical in most cases.
4. Load the sample into the column and collect the flow liquid, repeat this action for 3-5 times. If necessary, repeat for more times, then deal with the collected liquid reasonably.
5. Wash the column with Equilibration buffer B to remove other proteins.
6. Elute with Elution buffer, collect the flow liquid (antibody), adjust its pH by saturated Na2CO3 during collection. Then, customers can test the related data of the antibody as their own requirements. Re-equilibration anbuffer:
3. % glycerol
1. Add 5-10 mL Elution buffer to column to elute thoroughly, then neutralizate the column with Equilibration buffer A.
2. Wash the column bed with Storage buffer in 3-5 column volumes, seal the bottom of the column and store at -20 °C for at least one year. For frequent use, an aliquot can be stored at 0 °C for 1 month with addition of 0.001 % sodium azide (NaN3)
3. to the storage buffer.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Handling Advice Note: 20 % ethanol was contained as protection solution in this product, please wipe off the ethanol before use.
Storage 4 °C/-20 °C
Storage Comment At -20 °C for at least one year. For frequent use, an aliquot can be stored at 4 °C for 1 month with addition of 0. 02 % sodium azide (NaN3) to the storage buffer.
Expiry Date 12 months