|
Format
|
Lyophilized
|
|
Reconstitution
|
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives. Reconstitute immediately upon receipt! Avoid bright light to minimize photo bleeching of the fluorescent dye. Application Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µL for 30 min at RT in the dark. Adjust the antibody to the desired dilution and immediately use it for your internalization assay. ICC: image
|
|
Buffer
|
PBS
|
|
Handling Advice
|
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
|
|
Storage
|
-20 °C
|
|
Storage Comment
|
Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20 °C until use, or store aliquots at -80 °C without additives.
|
