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  • 产品名称：anti-alphaTubulinantibody(TUBA1)(AA419-435)(Oyster550)

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  • 产品厂商：SYSY
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简单介绍：
anti-alphaTubulinantibody(TUBA1)(AA419-435)(Oyster550)
详情介绍：
Immunogen Synthetic peptide SEAREDMAALEKDYEEV (aa 419-435 in human alpha-tubulin) coupled to key-hole limpet hemocyanin via an added N -terminal cysteine residue.
Clone 3A2
Isotype IgG1
Specificity Specific for alpha-tubulin (glu- and tyr-alpha-tubulin).
Purification purified IgG , fluorescence-labeled with Oyster? 550. Rabbit serum albumin was added for stabilization.
Alternative Name alpha-Tubulin (TUBA1 Antibody Abstract)
Research Area Cytoskeleton
Pathways Microtubule Dynamics
Application Notes WB: 1 : 1000 up to 1 : 50000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC-P: 1 : 200
Comment

ELISA: Suitable as capture antibodies for sandwich-ELISA with ABIN1742550 as detector antibody (protocol for sandwich-ELISA).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Add 1:1 (v/v) glycerol, then aliquot and store at -20 °C until use. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. The mounting agent Aquatex? (Merck Chemicals) is not compatible with Oyster dyes!
Buffer PBS
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1?mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Prolonged storage at 4 °C is not recommended!