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  • 产品名称：anti-CACNA1Bantibody(CalciumChannel,Voltage-Dependent,NType,alpha1BSubunit)(AA1

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  • 产品厂商：SYSY
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简单介绍：
anti-CACNA1Bantibody(CalciumChannel,Voltage-Dependent,NType,alpha1BSubunit)(AA1921-2222)
详情介绍：
Immunogen Recombinant protein (aa 1921-2222 of rat Ca2+-channel alpha-1E subunit).
Specificity Specific for Ca2+ channel alpha-1E.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Ca2+ Channel (CACNA1B Antibody Abstract)
Background Synonyms: Cav2.3
Application Notes WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500
Comment

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: IHC: This antibody requires a mild fixation protocol (1 % PFA in sodium acetate buffer, pH 6).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) (AA 1921-2222) antibody (ABIN1742328) dilution: 1 : 1000, sample: rat hippocampus lysate
Immunohistochemistry (IHC) image for anti-Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B) (AA 1921-2222) antibody (ABIN1742328) Indirect immunostaining of PFA fixed hippocampus section (dilution 1 : 500).