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  • 产品名称：anti-N-Ethylmaleimide-SensitiveFactorAttachmentProtein,gamma(NAPG)(AA2-185)anti

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  • 产品厂商：SYSY
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简单介绍：
anti-N-Ethylmaleimide-SensitiveFactorAttachmentProtein,gamma(NAPG)(AA2-185)antibody
详情介绍：
Immunogen Synthetic peptide AAQKINEGLEHLAKAEK (2-18 in rat gamma SNAP) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for gamma SNAP.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name gamma SNAP (NAPG Antibody Abstract)
Application Notes WB: 1 : 500 up to 1 : 2000 (AP staining)
ICC: not tested yet
IHC: not tested yet
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, gamma (NAPG) (AA 2-185) antibody (ABIN1742244) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Product cited in: de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." in: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, 2015 (PubMed).

Burgalossi, Jung, Meyer, Jockusch, Jahn, Taschenberger, OConnor, Nishiki, Takahashi, Brose, Rhee: "SNARE protein recycling by αSNAP and βSNAP supports synaptic vesicle priming." in: Neuron, Vol. 68, Issue 3, pp. 473-87, 2010 (PubMed).