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  • 产品名称：anti-Otoferlinantibody(OTOF)(Isoform1)

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  • 产品厂商：SYSY
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简单介绍：
anti-Otoferlinantibody(OTOF)(Isoform1)
详情介绍：
Immunogen Synthetic peptide GKTRSHKEEPQRQDEP (aa 196-211 in mouse isoform 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for otoferlin.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Otoferlin (OTOF Antibody Abstract)
Pathways Sensory Perception of Sound, Synaptic Vesicle Exocytosis
Application Notes WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 5000
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Immunohistochemistry (IHC) image for anti-Otoferlin (OTOF) (AA 196-211), (Isoform 1) antibody (ABIN1742371) Immunostaining of cochlear inner hair cells (IHCs) from p17 C57black mouse. Fixation ...
Product cited in: Vogl, Cooper, Neef, Wojcik, Reim, Reisinger, Brose, Rhee, Moser, Wichmann: "Unconventional molecular regulation of synaptic vesicle replenishment in cochlear inner hair cells." in: Journal of cell science, Vol. 128, Issue 4, pp. 638-44, 2015 (PubMed).