anti-Doc2a/bantibody 抗体网

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产品名称：anti-Doc2a/bantibody
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产品厂商：SYSY
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简单介绍：

anti-Doc2a/bantibody

详情介绍：

Product details
Product details
Immunogen	Recombinant full length rat Doc 2a protein.
Specificity	Detects preferentially Doc 2a
Cross-Reactivity (Details)	some cross-reactivity to Doc 2b.
Purification	Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Target details
Target details

Application Details
Application Details
Application Notes	WB: 1 : 1000 IP: not tested yet ICC: 1 : 100 up to 1 : 500 IHC: 1 : 200
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer	PBS
Handling Advice	Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage	-20 °C
Storage Comment	Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Images
Images
Supplier Images	dilution: 1 : 1000, sample: crude synaptic vesicle fraction of rat brain (LP2) Indirect immunostaining of rat hippocampus neurons (dilution 1 : 100). Immunostaining of a PFA fixed mouse hippocampus section with anti-Doc2a/b (dilution 1... Images for product: anti-Doc 2a/b antibody dilution: 1 : 1000, sample: crude synaptic vesicle fraction of rat brain (LP2) Indirect immunostaining of rat hippocampus neurons (dilution 1 : 100). Immunostaining of a PFA fixed mouse hippocampus section with anti-Doc2a/b (dilution 1 : 200; red) and Oyster? 650 labeled anti-calretinin (cat. no. 214 111C5, dilution 1 : 200; green). Nuclei have been visualized by DAPI staining (blue).

References
References
Product cited in:	Wilhelm, Mandad, Truckenbrodt, Kr?hnert, Sch?fer, Rammner, Koo, Cla?en, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, 2014 (PubMed). Schütz, Zilly, Lang, Jahn, Bruns: "A dual function for Munc-18 in exocytosis of PC12 cells." in: The European journal of neuroscience, Vol. 21, Issue 9, pp. 2419-32, 2005 (PubMed).