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  • 产品名称：anti-Unc-13HomologA(C.Elegans)(UNC13A)(AA3-317)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Unc-13HomologA(C.Elegans)(UNC13A)(AA3-317)antibody
详情介绍：
Immunogen Recombinant rat munc 13-1 (aa 3-317).
Specificity Specific for munc 13-1.
Purification antiserum
Alternative Name Munc 13-1 (UNC13A Antibody Abstract)
Pathways Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: 1 : 200
ELISA: not tested yet
Comment

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Unc-13 Homolog A (C. Elegans) (UNC13A) (AA 3-317) antibody (ABIN1742277) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Unc-13 Homolog A (C. Elegans) (UNC13A) (AA 3-317) antibody (ABIN1742277) Indirect immunostaining of PFA fixed mouse hippocampus neurons (dilution 1 : 500).
Immunohistochemistry (IHC) image for anti-Unc-13 Homolog A (C. Elegans) (UNC13A) (AA 3-317) antibody (ABIN1742277) Indirect immunostaining of a PFA fixed mouse hippocampus section (dilution 1 : 200; r...
Product cited in: Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, 2015 (PubMed).

Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." in: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, 2013 (PubMed).

Rinetti, Schweizer: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, 2010 (PubMed).