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  • 产品名称:anti-Syntaxin4(STX4)(AA1-273),(CytoplasmicDomain)antibody

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简单介绍:
anti-Syntaxin4(STX4)(AA1-273),(CytoplasmicDomain)antibody
详情介绍:
Immunogen Recombinant cytoplasmic domain of rat syntaxin 4 (aa 1-273).
Specificity Specific for syntaxin 4.
Purification antiserum
Alternative Name Syntaxin 4 (STX4 Antibody Abstract)
Research Area Signaling
Pathways Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 100
IHC: not tested yet
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Syntaxin 4 (STX4) (AA 1-273), (Cytoplasmic Domain) antibody (ABIN1742221) dilution: 1 : 5000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Syntaxin 4 (STX4) (AA 1-273), (Cytoplasmic Domain) antibody (ABIN1742221) Indirect immunostaining of HELA cells (dilution 1 : 100; green). Nuclei have been vis...
Product cited in: Arasaki, Roy: "Legionella pneumophila promotes functional interactions between plasma membrane syntaxins and Sec22b." in: Traffic (Copenhagen, Denmark), Vol. 11, Issue 5, pp. 587-600, 2010 (PubMed).

Bajohrs, Darios, Peak-Chew, Davletov: "Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell." in: The Biochemical journal, Vol. 392, Issue Pt 2, pp. 283-9, 2005 (PubMed).

Li, Alexander, Schwartz: "Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 19791-7, 2003 (PubMed).

Toyomura, Murata, Yamamoto, Oka, Sun-Wada, Wada, Futai: "From lysosomes to the plasma membrane: localization of vacuolar-type H+ -ATPase with the a3 isoform during osteoclast differentiation." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 22023-30, 2003 (PubMed).

Riedel, Antonin, Fernandez-Chacon, Alvarez de Toledo, Jo, Geppert, Valentijn, Valentijn, Jamieson, Südhof, Jahn: "Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6487-97, 2002 (PubMed).