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  • 产品名称：anti-ProtonATPase(AA826-838)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-ProtonATPase(AA826-838)antibody
详情介绍：
Immunogen Synthetic peptide FSFEHIREGKFDE (826-838 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for the alpha1 116kDa subunit.
Purification antiserum
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100
IHC-P: 1 : 100
Comment

WB: The proton pump aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. ICC: Methanol fixation is recommended.
Restrictions For Research Use only
Format Liquid
Buffer PBS
Handling Advice Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4?°C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20?°C),however, is preferable.
Storage 4 °C/-20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Proton ATPase (AA 826-838) antibody (ABIN1742216) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Proton ATPase (AA 826-838) antibody (ABIN1742216) Indirect immunostaining of methanol fixed rat hippocampus neurons (dilution 1 : 100; ...
Immunohistochemistry (IHC) image for anti-Proton ATPase (AA 826-838) antibody (ABIN1742216) anti-Proton ATPase (AA 826-838) antibody (Image 3)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Proton ATPase (AA 826-838) antibody (ABIN1742216) Indirect immunostaining of paraffin embedded mouse brain section with anti-v-ATPase (...
Product cited in: Gouzy, Larrouy-Maumus, Bottai, Levillain, Dumas, Wallach, Caire-Brandli, de Chastellier, Wu, Poincloux, Brosch, Guerquin-Kern, Schnappinger, Sório de Carvalho, Poquet, Neyrolles: "Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003928, 2014 (PubMed).

Yao, de la Iglesia, Bajjalieh: "Loss of the SV2-like protein SVOP produces no apparent deficits in laboratory mice." in: PLoS ONE, Vol. 8, Issue 7, pp. e68215, 2013 (PubMed).