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  • 产品名称：anti-GFPantibody(GreenFluorescentProtein)

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  • 产品厂商：SYSY
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简单介绍：
anti-GFPantibody(GreenFluorescentProtein)
详情介绍：
Immunogen Strep-Tag? fusion protein, full-length GFP.
Cross-Reactivity (Details) Cross reacts with all GFP derivatives tested so far (e.g. EYFP, ECFP, EGFP).
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name GFP
Research Area Tags/Labels
Application Notes WB: 1 : 1000 up to 1 : 20000 (AP staining)
IP: not tested yet
ICC: 1 : 100 up to 1 : 1000
IHC-P: not tested yet
Comment

ABIN1742297 Suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1742293) dilution: 1 : 1000, sample: total cell lysate of GFP transfected fibroblasts
image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1742293) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
Product cited in: Rotmann, Strand, Martiné, Closs: "Protein kinase C activation promotes the internalization of the human cationic amino acid transporter hCAT-1. A new regulatory mechanism for hCAT-1 activity." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54185-92, 2004 (PubMed).