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  • 产品名称:anti-SNAP91antibody(Synaptosomal-AssociatedProtein,91kDaHomolog(Mouse))(AA279-2

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  • 产品厂商:SYSY
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简单介绍:
anti-SNAP91antibody(Synaptosomal-AssociatedProtein,91kDaHomolog(Mouse))(AA279-297)
详情介绍:
Immunogen Synthetic peptide (aa 279-297) of rat AP 180 coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for AP 180.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name AP 180 (SNAP91 Antibody Abstract)
Background Synonyms: pp155, NP185, F1-20, AP-3, SNAP 91
Application Notes WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 1000
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Synaptosomal-Associated Protein, 91kDa Homolog (Mouse) (SNAP91) (AA 279-297) antibody (ABIN1742332) dilution: 1 : 1000, sample: crude synaptic vesicle fraction of rat brain (LP2)
Immunocytochemistry (ICC) image for anti-Synaptosomal-Associated Protein, 91kDa Homolog (Mouse) (SNAP91) (AA 279-297) antibody (ABIN1742332) Indirect immunofluorescence labeling of PFA fixed rat hippocampus neurons (dilution 1...
Immunohistochemistry (IHC) image for anti-Synaptosomal-Associated Protein, 91kDa Homolog (Mouse) (SNAP91) (AA 279-297) antibody (ABIN1742332) Presynaptic immunostaining of the calyx of Held in the medial nucleus of the trapezoi...
Product cited in: Fuchs, Brandst?tter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, 2014 (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kr?hnert, Sch?fer, Rammner, Koo, Cla?en, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, 2014 (PubMed).

Geis, Weishaupt, Hallermann, Grünewald, Wessig, Wultsch, Reif, Byts, Beck, Jablonka, Boettger, ü?eyler, Fouquet, Gerlach, Meinck, Sirén, Sigrist, Toyka, Heckmann, Sommer: "Stiff person syndrome-associated autoantibodies to amphiphysin mediate reduced GABAergic inhibition." in: Brain : a journal of neurology, Vol. 133, Issue 11, pp. 3166-80, 2010 (PubMed).

Zander, Münster-Wandowski, Brunk, Pahner, Gómez-Lira, Heinemann, Gutiérrez, Laube, Ahnert-Hilger: "Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 22, pp. 7634-45, 2010 (PubMed).