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  • 产品名称：anti-Syntaxin4(STX4)(AA1-273),(CytoplasmicDomain)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Syntaxin4(STX4)(AA1-273),(CytoplasmicDomain)antibody
详情介绍：
Immunogen Recombinant cytoplasmic domain of rat syntaxin 4 (aa 1-273).
Specificity Specific for syntaxin 4.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Syntaxin 4 (STX4 Antibody Abstract)
Research Area Signaling
Pathways Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 100
IHC: not tested yet
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Syntaxin 4 (STX4) (AA 1-273), (Cytoplasmic Domain) antibody (ABIN1742222) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-Syntaxin 4 (STX4) (AA 1-273), (Cytoplasmic Domain) antibody (ABIN1742222) Indirect immunostaining of Hela cells with anti-syntaxin 4 (dilution 1 : 100; red) an...
Product cited in: Tao-Cheng, Pham, Yang, Winters, Gallant, Reese: "Syntaxin 4 is concentrated on plasma membrane of astrocytes." in: Neuroscience, Vol. 286, pp. 264-71, 2015 (PubMed).

Ulloa, Gonzàlez-Juncà, Meffre, Barrecheguren, Martínez-Mármol, Pazos, Olivé, Cotrufo, Seoane, Soriano: "Blockade of the SNARE protein syntaxin 1 inhibits glioblastoma tumor growth." in: PLoS ONE, Vol. 10, Issue 3, pp. e0119707, 2015 (PubMed).

Werno, Chamberlain: "S-acylation of the Insulin-Responsive Aminopeptidase (IRAP): Quantitative analysis and Identification of Modified Cysteines." in: Scientific reports, Vol. 5, pp. 12413, 2015 (PubMed).

Baron, Ozgen, Klunder, de Jonge, Nomden, Plat, Trifilieff, de Vries, Hoekstra: "The major myelin-resident protein PLP is transported to myelin membranes via a transcytotic mechanism: involvement of sulfatide." in: Molecular and cellular biology, Vol. 35, Issue 1, pp. 288-302, 2014 (PubMed).