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  • 产品名称:anti-Rabphilin3AHomolog(Mouse)(RPH3A)(AA671-684)antibody

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简单介绍:
anti-Rabphilin3AHomolog(Mouse)(RPH3A)(AA671-684)antibody
详情介绍:
Immunogen Synthetic peptide WHQLQNENHVSSD (aa 671-684 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for rabphilin 3a.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Rabphilin 3a (RPH3A Antibody Abstract)
Background Synonyms: Exophilin 1
Pathways Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 (AP staining)
ICC: not tested yet
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in Tris buffer. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Rabphilin 3A Homolog (Mouse) (RPH3A) (AA 671-684) antibody (ABIN1742255) dilution: 1 : 1000, sample: crude synaptic vesicle fraction of rat brain (LP2)
Product cited in: Morciano, Beckhaus, Karas, Zimmermann, Volknandt: "The proteome of the presynaptic active zone: from docked synaptic vesicles to adhesion molecules and maxi-channels." in: Journal of neurochemistry, Vol. 108, Issue 3, pp. 662-75, 2009 (PubMed).

Yang, Farias, Kapfhamer, Tobias, Grant, Abel, Bućan: "Biochemical, molecular and behavioral phenotypes of Rab3A mutations in the mouse." in: Genes, brain, and behavior, Vol. 6, Issue 1, pp. 77-96, 2007 (PubMed).