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  • 产品名称：anti-RAB3A,MemberRASOncogeneFamily(RAB3A)(AA154-227)antibody

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简单介绍：
anti-RAB3A,MemberRASOncogeneFamily(RAB3A)(AA154-227)antibody
详情介绍：
Immunogen Recombinant human Rab 3c (aa 154-227).
Specificity Specific for all four Rab 3 isoforms a, b, c, d.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Rab 3 (RAB3A Antibody Abstract)
Pathways Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
Application Notes WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC: 1 : 200 up to 1 : 500
Comment

This antibody has been affinity purified with the complete antigen (aa 154 - 227) and recognizes all isoforms, due to the homology of Rab 3 proteins.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-RAB3A, Member RAS Oncogene Family (RAB3A) (AA 154-227) antibody (ABIN1742210) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-RAB3A, Member RAS Oncogene Family (RAB3A) (AA 154-227) antibody (ABIN1742210) Immunocytochemistry on cultured hippocampus neurons (dilution 1 : 1000). Immunoreacti...
Immunohistochemistry (IHC) image for anti-RAB3A, Member RAS Oncogene Family (RAB3A) (AA 154-227) antibody (ABIN1742210) Indirect immunolabeling of PFA fixed mouse spinal chord vibratome section (dilution 1...
Product cited in: Romanov, Alpár, Zhang, Zeisel, Calas, Landry, Fuszard, Shirran, Schnell, Dobolyi, Oláh, Spence, Mulder, Martens, Palkovits, Uhlen, Sitte, Botting, Wagner, Linnarsson, H?kfelt, Harkany: "A secretagogin locus of the mammalian hypothalamus controls stress hormone release." in: The EMBO journal, Vol. 34, Issue 1, pp. 36-54, 2015 (PubMed).

Spangler, Schmitz, Kevenaar, de Graaff, de Wit, Demmers, Toonen, Hoogenraad: "Liprin-α2 promotes the presynaptic recruitment and turnover of RIM1/CASK to facilitate synaptic transmission." in: The Journal of cell biology, Vol. 201, Issue 6, pp. 915-28, 2013 (PubMed).

de Wit, Walter, Milosevic, Gulyás-Kovács, Riedel, S?rensen, Verhage: "Synaptotagmin-1 docks secretory vesicles to syntaxin-1/SNAP-25 acceptor complexes." in: Cell, Vol. 138, Issue 5, pp. 935-46, 2009 (PubMed).