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  • 产品名称:anti-Rab3cantibody(RAB3C,MemberRASOncogeneFamily)(AA154-227)

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简单介绍:
anti-Rab3cantibody(RAB3C,MemberRASOncogeneFamily)(AA154-227)
详情介绍:
Immunogen Recombinant protein of human Rab 3c (aa 154-227).
Specificity Specific for Rab 3c.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Rab 3c (Rab3c Antibody Abstract)
Application Notes WB: 1 : 1000 (AP staining)
ICC: 1 : 500
IHC: not tested yet
Comment

This antibody has been affinity purified with a peptide consisting only of the Rab 3c specific epitope NTRLKETPPPPQPN (aa 211 - 224) of the human protein.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Immunocytochemistry (ICC) image for anti-RAB3C, Member RAS Oncogene Family (Rab3c) (AA 154-227) antibody (ABIN1742212) Immunocytochemistry on cultured hippocampus neurons (dilution 1 : 500). Immunoreactiv...
Western Blotting (WB) image for anti-RAB3C, Member RAS Oncogene Family (Rab3c) (AA 154-227) antibody (ABIN1742212) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Product cited in: Nishioka, Tooi, Isobe, Nakatsuji, Aiba: "BMS-708163 and Nilotinib restore synaptic dysfunction in human embryonic stem cell-derived Alzheimer's disease models." in: Scientific reports, Vol. 6, pp. 33427, 2016 (PubMed).

Pavlos, Gr?nborg, Riedel, Chua, Boyken, Kloepper, Urlaub, Rizzoli, Jahn: "Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 40, pp. 13441-53, 2010 (PubMed).

Cai, Reim, Varoqueaux, Tapechum, Hill, S?rensen, Brose, Chow: "Complexin II plays a positive role in Ca2+-triggered exocytosis by facilitating vesicle priming." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 49, pp. 19538-43, 2008 (PubMed).