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  • 产品名称：anti-VAMP5antibody(Vesicle-AssociatedMembraneProtein5(Myobrevin))(AA1-70)

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简单介绍：
anti-VAMP5antibody(Vesicle-AssociatedMembraneProtein5(Myobrevin))(AA1-70)
详情介绍：
Immunogen Recombinant mouse myobrevin (aa 1-70).
Specificity Specific for myobrevin.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Myobrevin (VAMP5 Antibody Abstract)
Background Synonyms: VAMP 5
Application Notes WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 1000
IHC-P: 1 : 200
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5) (AA 1-70) antibody (ABIN1742369) dilution: 1 : 1000
Immunocytochemistry (ICC) image for anti-Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5) (AA 1-70) antibody (ABIN1742369) Indirect immunolabeling of PFA fixed C2C12 cells differentiated into myotubes (red, d...
Immunohistochemistry (IHC) image for anti-Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5) (AA 1-70) antibody (ABIN1742369) anti-Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5) (AA 1-70) antibody (Image 3)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Vesicle-Associated Membrane Protein 5 (Myobrevin) (VAMP5) (AA 1-70) antibody (ABIN1742369) Indirect immunostaining of paraffin embedded sections of mouse skeletal muscle (1 : 2...
Product cited in: Sadler, Bryant, Gould: "Characterization of VAMP isoforms in 3T3-L1 adipocytes: implications for GLUT4 trafficking." in: Molecular biology of the cell, Vol. 26, Issue 3, pp. 530-6, 2015 (PubMed).

Takahashi, Tajika, Khairani, Ueno, Murakami, Yorifuji: "The localization of VAMP5 in skeletal and cardiac muscle." in: Histochemistry and cell biology, Vol. 139, Issue 4, pp. 573-82, 2013 (PubMed).

Rose, Jeppesen, Kiens, Richter: "Effects of contraction on localization of GLUT4 and v-SNARE isoforms in rat skeletal muscle." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 297, Issue 5, pp. R1228-37, 2009 (PubMed).