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  • 产品名称：anti-DNAJC5antibody(DnaJ(Hsp40)Homolog,SubfamilyC,Member5)(C-Term)

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简单介绍：
anti-DNAJC5antibody(DnaJ(Hsp40)Homolog,SubfamilyC,Member5)(C-Term)
详情介绍：
Immunogen Synthetic peptide of rat CSP (C-terminal part, aa 182-198) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Specificity Specific for CSP.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name CSP (DNAJC5 Antibody Abstract)
Research Area Neurology
Pathways Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 100 up to 1 : 1000
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5) (AA 182-198), (C-Term) antibody (ABIN1742330) dilution: 1 : 1000, sample: crude synaptosomal fraction of rat brain (P2)
Immunocytochemistry (ICC) image for anti-DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5) (AA 182-198), (C-Term) antibody (ABIN1742330) Indirect immunolabeling of cultured rat hippocampus neurons (dilution 1 : 500).
Immunohistochemistry (IHC) image for anti-DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5) (AA 182-198), (C-Term) antibody (ABIN1742330) Immunostaining of cochlear inner hair cells from 3 weeks old rats.
Product cited in: Wilhelm, Mandad, Truckenbrodt, Kr?hnert, Sch?fer, Rammner, Koo, Cla?en, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, 2014 (PubMed).

Sharrad, Gai, Brookes et al.: "Selective coexpression of synaptic proteins, ?-synuclein, cysteine string protein-?, synaptophysin, synaptotagmin-1, and synaptobrevin-2 in vesicular acetylcholine transporter-immunoreactive axons in ..." in: The Journal of comparative neurology, Vol. 521, Issue 11, pp. 2523-37, 2013 (PubMed).

Lim, Kehm, Lee, Soper, Li, Trojanowski, Lee: "α-Syn suppression reverses synaptic and memory defects in a mouse model of dementia with Lewy bodies." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 27, pp. 10076-87, 2011 (PubMed).

García-Junco-Clemente, Cantero, Gómez-Sánchez, Linares-Clemente, Martínez-López, Luján, Fernández-Chacón: "Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7377-91, 2010 (PubMed).

Wahlin, Moreira, Huang, Yu, Adler: "Molecular dynamics of photoreceptor synapse formation in the developing chick retina." in: The Journal of comparative neurology, Vol. 506, Issue 5, pp. 822-37, 2007 (PubMed).