anti-Abeta-pE3antibody 抗体网

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产品名称：anti-Abeta-pE3antibody
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产品厂商：SYSY
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简单介绍：

anti-Abeta-pE3antibody

详情介绍：

Product details
Product details
Immunogen	Synthetic peptide pyr-EFRHD coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
Specificity	Specific for Abeta-pE3.
Purification	Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Target details
Target details
Background	Synonyms: pyro-Glu Abeta, pE-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42)

Application Details
Application Details
Application Notes	WB: 1 : 1000 IP: not tested yet ICC: not tested yet IHC: 1 : 500 IHC-P: 1 : 200
Comment	WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer	PBS
Handling Advice	Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage	-20 °C
Storage Comment	Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Images
Images
Supplier Images	Detection of different synthetic Abeta species (dilution 1 : 1000). Peptides have bee... Immunostaining of a PFA fixed brain section from a 5XFAD mouse (dilution 1 : 500). Immunostaining of paraffin embedded sections from an Alzheimer's patient (dilution 1 ... Images for product: anti-Abeta-pE3 antibody Detection of different synthetic Abeta species (dilution 1 : 1000). Peptides have been resolved by denaturing 16.5 % Schaegger PAGE. Immunostaining of a PFA fixed brain section from a 5XFAD mouse (dilution 1 : 500). Immunostaining of paraffin embedded sections from an Alzheimer's patient (dilution 1 : 200). Note accumulation of pyro-Glu Abeta in plaques. Immunoreactivity was revealed using diaminobenzidine as chromagen. Nuclei were counterstained with haematoxylin (blue).

References
References
Product cited in:	Morawski, Schilling, Kreuzberger, Waniek, J?ger, Koch, Cynis, Kehlen, Arendt, Hartlage-Rübsamen, Demuth, Ro?ner: "Glutaminyl cyclase in human cortex: correlation with (pGlu)-amyloid-? load and cognitive decline in Alzheimer's disease." in: Journal of Alzheimer's disease : JAD, Vol. 39, Issue 2, pp. 385-400, 2014 (PubMed). Becker, Kohlmann, Alexandru, Jagla, Canneva, B?uscher, Cynis, Sedlmeier, Graubner, Schilling, Demuth, von H?rsten: "Glutaminyl cyclase-mediated toxicity of pyroglutamate-beta amyloid induces striatal neurodegeneration." in: BMC neuroscience, Vol. 14, pp. 108, 2013 (PubMed). Nussbaum, Schilling, Cynis, Silva, Swanson, Wangsanut, Tayler, Wiltgen, Hatami, R?nicke, Reymann, Hutter-Paier, Alexandru, Jagla, Graubner, Glabe, Demuth, Bloom: "Prion-like behaviour and tau-dependent cytotoxicity of pyroglutamylated amyloid-β." in: Nature, Vol. 485, Issue 7400, pp. 651-5, 2012 (PubMed). Morawski, Brückner, J?ger, Seeger, Matthews, Arendt: "Involvement of perineuronal and perisynaptic extracellular matrix in Alzheimer's disease neuropathology." in: Brain pathology (Zurich, Switzerland), Vol. 22, Issue 4, pp. 547-61, 2012 (PubMed). Hartlage-Rübsamen, Morawski, Waniek, J?ger, Zeitschel, Koch, Cynis, Schilling, Schliebs, Demuth, Rossner: "Glutaminyl cyclase contributes to the formation of focal and diffuse pyroglutamate (pGlu)-A? deposits in hippocampus via distinct cellular mechanisms." in: Acta neuropathologica, Vol. 121, Issue 6, pp. 705-19, 2011 (PubMed). Alexandru, Jagla, Graubner, Becker, B?uscher, Kohlmann, Sedlmeier, Raber, Cynis, R?nicke, Reymann, Petrasch-Parwez, Hartlage-Rübsamen, Waniek, Rossner, Schilling, Osmand, Demuth, von H?rsten: "Selective hippocampal neurodegeneration in transgenic mice expressing small amounts of truncated Aβ is induced by pyroglutamate-Aβ formation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 36, pp. 12790-801, 2011 (PubMed).