anti-GADantibody(GlutamateDecarboxylase1(Brain,67kDa))(AA3-101) 抗体网

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产品名称：anti-GADantibody(GlutamateDecarboxylase1(Brain,67kDa))(AA3-101)
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产品厂商：SYSY
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简单介绍：

anti-GADantibody(GlutamateDecarboxylase1(Brain,67kDa))(AA3-101)

详情介绍：

Product Details anti-GAD Antibody
Product Details anti-GAD Antibody
Immunogen	Recombinant mouse GAD 1 (aa 3-101).
Specificity	Specific for GAD 1 / GAD 67.
Purification	Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Target Details GAD
Target Details GAD
Alternative Name	GAD 1 (GAD1 Antibody Abstract)
Background	Synonyms: GAD 67

Application Details
Application Details
Application Notes	WB: not recommended IP: not tested yet ICC: 1 : 500 IHC: 1 : 200 up to 1 : 500
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer	PBS
Handling Advice	Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage	-20 °C
Storage Comment	Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Images
Images
Supplier Images	Immunolabeling of cultured hippocampus neurons (dilution 1 : 500). A: Indirect immunolabeling of PFA fixed rat hippocampus sections. B: Indirect immuno... Immunostaining of PFA fixed, paraffin embedded section of mouse cerebellum (dilution ... Images for product: anti-Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 3-101) antibody Immunolabeling of cultured hippocampus neurons (dilution 1 : 500). A: Indirect immunolabeling of PFA fixed rat hippocampus sections. B: Indirect immunohystochemistry of a rat cerebellum section (dilution 1 : 500). GAD1 positive structures were revealed with a biotinylated goat anti-rabbit secondary antibody in combination with peroxidase coupled streptavidin. Nickle amplified diaminobenzidine was used as chromagen. Immunostaining of PFA fixed, paraffin embedded section of mouse cerebellum (dilution 1 : 1000). Immunoreactivity was revealed using diaminobenzidine as chromagen. Nuclei have been counterstained with haematoxylin (blue).

References for anti-GAD antibody (ABIN1742416)
References for anti-GAD antibody (ABIN1742416)
Product cited in:	Heinrich, Blum, Gascón, Masserdotti, Tripathi, Sánchez, Tiedt, Schroeder, G?tz, Berninger: "Directing astroglia from the cerebral cortex into subtype specific functional neurons." in: PLoS biology, Vol. 8, Issue 5, pp. e1000373, 2010 (PubMed).