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  • 产品名称：anti-Synaptojanin1(SYNJ1)(AA1292-1308),(Isoform2)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-Synaptojanin1(SYNJ1)(AA1292-1308),(Isoform2)antibody
详情介绍：
Immunogen Synthetic peptide CLPSDSSPQLQQEQP (aa 1292-1308 in isoform 2) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Recognizes all isoforms of synaptojanin 1.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name Synaptojanin 1 (SYNJ1 Antibody Abstract)
Pathways Inositol Metabolic Process, Synaptic Vesicle Exocytosis
Application Notes WB: 1 : 200 up to 1 : 2000 (AP staining)
IP: not tested yet
Comment

Running SDS-PAGE with non-boiled samples gives stronger signals.
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-Synaptojanin 1 (SYNJ1) (AA 1292-1308), (Isoform 2) antibody (ABIN1742316) dilution: 1 : 200, sample: crude synaptosomal fraction of rat brain (P2)
Product cited in: Cliburn, Dunn, Stout, Hoffman, Lohr, Bernstein, Winokur, Burkett, Schmitz, Caudle, Miller: "Immunochemical localization of vesicular monoamine transporter 2 (VMAT2) in mouse brain." in: Journal of chemical neuroanatomy, 2016 (PubMed).

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, 2014 (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, 2014 (PubMed).

Park, Kim, Lee, Park, Park, Sun, Kim, Chang: "SNX18 shares a redundant role with SNX9 and modulates endocytic trafficking at the plasma membrane." in: Journal of cell science, Vol. 123, Issue Pt 10, pp. 1742-50, 2010 (PubMed).

Uthaiah, Hudspeth: "Molecular anatomy of the hair cell's ribbon synapse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12387-99, 2010 (PubMed).

Hoopmann, Punge, Barysch, Westphal, Bückers, Opazo, Bethani, Lauterbach, Hell, Rizzoli: "Endosomal sorting of readily releasable synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 44, pp. 19055-60, 2010 (PubMed).

Shin, Lee, Ahn, Kim, Ahn, YongPark, Chang: "Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis." in: The Journal of biological chemistry, Vol. 282, Issue 39, pp. 28939-50, 2007 (PubMed).