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  • 产品名称：anti-NMDAReceptor1(AA35-53)antibody

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  • 产品厂商：SYSY
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简单介绍：
anti-NMDAReceptor1(AA35-53)antibody
详情介绍：
Immunogen Synthetic peptide (aa 35-53 of rat GluN 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for GluN 1.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name NMDA-Receptor 1
Application Notes WB: 1 : 1000 (AP staining)
ICC: not recommended
IHC: not recommended
Comment

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. IHC: Antibody 2, ABIN1742247, is recommended for IHC. ELISA: Suitable as detector antibody for sandwich-ELISA as capture antibody (protocol for sandwich-ELISA).
Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Western Blotting (WB) image for anti-NMDA Receptor 1 (AA 35-53) antibody (ABIN1742246) dilution: 1 : 1000, sample: synaptic membrane fraction of rat brain (LP1)
Product cited in: Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." in: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, 2012 (PubMed).

Ribic, Flügge, Schlumbohm, M?tz-Rensing, Walter, Fuchs: "Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey." in: Behavioral and brain functions : BBF, Vol. 7, pp. 1, 2011 (PubMed).