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  • 产品名称:anti-NMDAReceptor1(AA35-53)antibody

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  • 产品厂商:SYSY
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简单介绍:
anti-NMDAReceptor1(AA35-53)antibody
详情介绍:
Immunogen Synthetic peptide (aa 35-53 of rat GluN 1) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.
Specificity Specific for GluN 1.
Purification Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Alternative Name NMDA-Receptor 1
Application Notes WB: not recommended
IP: not tested yet
ICC: not recommended
IHC: 1 : 500 up to 1 : 1000
Comment

WB: Antibody 1, ABIN1742246, is recommended for WB. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008). Cell-type-dependent molecular composition of the axon initial segment. Journal of Neuroscience 28: 14329-40.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 50 μL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS
Handling Advice Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4?°C for prolonged periods (i.e. several weeks), is not recommended.
Storage -20 °C
Storage Comment Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Supplier Images
Immunohistochemistry (IHC) image for anti-NMDA Receptor 1 (AA 35-53) antibody (ABIN1742247) Indirect immunolabeling of PFA fixed rat hippocampus (CA1) treated with pepsin prior ...
Product cited in: Prüss, Leubner, Wenke, Czirják, Szentiks, Greenwood: "Anti-NMDA Receptor Encephalitis in the Polar Bear (Ursus maritimus) Knut." in: Scientific reports, Vol. 5, pp. 12805, 2015 (PubMed).